natural compounds found in medicinal plant of epilobium frigidum extracts by chromatography method

Eabstractepilobium genus has anti-inflammatory, antioxidant, antimicrobial, anti-tumor, anti-pain, and anti-androgenic medicinal properties in traditional medicine. in this research, the aerial parts of e. frigidum species were collected at the flowering stage from the heights of kalardasht in mazandaran province at the end of may and were phytochemically investigated for the composition of fatty acids and volatile active substances in the essential oil. the extract of plant sample was extracted with 80% methanol and evaluated by gas chromatography coupled with a mass spectrometer (gc-ms). the results showed that the main hydrocarbon compounds in the essential oil of this plant were linoleic acid with a value of 49.176 and oleic acid (32.227%) and volatile compounds 4aβ,7α,7aβ-nepetalactone with a value of 41.312 and β-caryophyllene (15.395). the chemical structure of linoleic acid, a common omega-6 fatty acid found in many nuts, seeds, and vegetable oils. hydrocarbon terpenes had the highest concentration in the essential oil of this plant. the results of gc/ms analysis showed the presence of 24 compounds in the investigated species, which were separated into the groups of steroids and terpenes from the phytochemical point of view. the findings showed that e. frigidum contains important natural products such as steroids, monoterpenoids, and sesquiterpenoids. due to the bioactive products, this plant can have more applications in the cosmetic and pharmaceutical industries.