optimize the synthesis of calcium hydroxide/levan/silver nanocomposite as an antibacterial agent

Antimicrobial resistance (amr) has become a worrisome problem due to the rapid increase in amr phenotypes. this research aimed to investigate the antibacterial properties of calcium hydroxide/levan/silver nanocomposite against enterococcus faecalis (e. faecalis) biofilm under in vitro conditions. in this study, nine experiments were designed to evaluate the antibacterial effects of synthesized nanocomposites against the oral pathogen e. faecalis using the taguchi method. in these tests, three factors, which included calcium hydroxide nanoparticles, biopolymer levan and silver nanoparticles, were investigated at three different levels to determine the best ratio with the highest antibacterial activity. the nanocomposite synthesized under test condition 5, including calcium hydroxide (50 mg/ml), levan (0.5 mg/ml) and silver (4 mg/ml), completely inhibited the growth of e. faecalis bacteria. analysis showed that the nanocomposite and its components had a desirable structural and chemical composition, making it an effective antibacterial agent.