synthesis, characterization of ethyl dioxoisoindolinyl cyclohexenone carboxylate derivatives from some chalcones and its biological activity assessment

In this study, new ethyl dioxoisoindolinyl cyclohexenone carboxylate derivatives have been synthesized via addition and cyclocondensation reactions when ethylacetoacetate added to chalcone compounds in a strong alkaline media. ethylacetoacetate was added to chalcone compounds (d1-d10) in ethanol as solvent to produce cyclohexenone compounds (d11-d20), using sodium hydroxide as catalyst. the prepared compounds structures have been identified using the infrared spectroscopy (ft-ir) and some of them by 1h-nmr and 13c-nmr spectroscopy. the chalcones were prepared from our earliest published work. these new cyclohexenones have been applied as antibacterial agent towards staphylococcus aureus, staphylococcus epidermidis (gram-positive bacteria), and escherichia coli (gram-negative bacteria). the results exhibited good antibacterial activity of the synthesized compounds against staphylococcus aureus and staphylococcus epidermidis at high concentrations (0.01 and 0.001) mg/ml compared to low concentration (0.001 mg/ml) because of high concentration effect.