validation of a rapid and sensitive reversed-phase liquid chromatography method and force degradation study of synthesized (e)-3-(2,4,6-tri-methoxyphenyl)-1-phenylpro-2 ene-1-one

A stability-indicating rp-hplc method was developed and validated for the determination of the estimation of newly synthesized (e)-3-(2,4,6-tri-methoxyphenyl)-1-phenylpro-2 ene-1-one in api form by using c18 column phenomenix (250 mm x4.6 mm,5μm), with a mobile phase consisting of a acetonitrile and methanol (40:60 v/v) with flow rate of 0.7 ml/min. the detection was carried out at 370 nm and retention time (rt) of (e)-3-(2, 4, 6- tri-methoxyphenyl)-1-phenylpro-2 ene-1-one was found to be 2.55 min. linearity was observed from the concentration range of 10-50μg/ml (n=5), (coefficient of determination r² was 0.997) with the equation of y= 0.495x + 0.111. the method was repeatable with a relative standard deviation (rsd) of 0.16 to 1.98 %. the newly developed method was validated according to the ich guidelines with respect to specificity, linearity, accuracy, precision and robustness. (e)-3-(2,4,6-tri-methoxyphenyl)-1-phenylpro-2 ene-1-one was subjected to different stress conditions as per ich guidelines like acidic alkaline, oxidative, thermal and the results showed that it was more sensitive towards oxidative.