antioxidant potential of secondary metabolites isolated from macaranga hypoleuca

Macaranga hypoleuca is a member of the euphorbiaceae family and demonstrates therapeutic properties. its leaves contain secondary metabolites, such as terpenoids, tannins, and phenolic compounds. this study aimed to extract secondary metabolites from the ethyl acetate fraction of m. hypoleuca leaves and assess their antioxidant properties. antioxidant testing of the ethyl acetate fraction via dpph, frap, and abts methods resulted in ic50 values of 3.861, 2.0864, and 2.707 µg/ml, respectively. the leaves were isolated through methanol maceration and further partitioned with n-hexane, ethyl acetate, and butanol. the ethyl acetate fraction underwent gravity column chromatography, identifying the primary isolate in fraction f10. spectral data from nmr and corroborative uv-vis and ftir analyses suggest the isolate f10e is quercitrin (quercetin-3-rhamnoside), with an antioxidant capacity yielding an ic50 of 10.41 µg/ml via the dpph method.