development of a new methodology for lincomycin.hci (lino.hci) determination using phosphomolybdic acid (pma) as a reagent and isnag-fluorimeter instrument via cfia

The reaction is based on the lincomycin that has been responded. in an acidic solution, combine hcl and phosphomolybdic acid to produce a white color precipitate that may be measured using an isnag-fluorimeter (homemade instrument). some chemical and physical variables have been evaluated. the correlation coefficient was r=0.9983. the calibration curve was linear (the range was 0.0051 mmol/l). the minimum detection limit concentration in the linear dynamic range of the calibration curve was 19.935 ng. the rsd percent of lincomycin hcl at 0.3 and 0.8 mmol/l was less than 0.25% (n=8). the approach was used to determine lincomycin hcl in three pharmaceutical tablets. comparisons were made using a standard expansion approach and a matched t-test between the recently created method and the traditional strategy (uv-vis spectrophotometry and turbidimetry with a turbidimeter). at the 95% confidence level, there was no significant difference between the two techniques.