survey of frequency of bla^per gene in p. aeruginosa by kirby bauer& pcr method

Aim: the present study was aimed to investigate frequency of bla per gene in hospital isolates of p. aeruginosa. methods: the number of 100 isolates of p. aeruginosa were isolated from in burn hospital in tehran during a 1-year study in 2013-2014.the identification were carried out by colonial morphology, pigment formation, positive oxidase test,; growth test at 42°c on nutrient agar, of, arginine dehyrolase and motility tests. results: the results indicated that 15% of burned hospital isolates contain bla pe gene. this study indicated that zone diameter mean of growth inhibition ≤ 14 mm to ceftazidime.the rate of mic to ceftazidime was 20 μg/ml. conclusion: according to results of current research we hope in future be used drugs to the clinic with a wider range as a complementary therapy and also for burned infections.