sero-prevalence of brucellosis in humans population of charsasdda, khyber pakhtunkhwa, pakistan

Background: brucellosis caused by brucella spp, is an infectious and zoonotic disease of wild and domestic animals of worldwide distribution. aim: the present research was conducted to determine the sero-prevalence of brucellosis in humans, homo sapien (l, 1758) population in charsadda, khyber pakhtunkhwa (kp), pakistan during august 2014-january 2015. methods: blood samples (n=200) were collected randomly during february-december 2014. they were screened for brucellosis by using serum plate agglutination test (spat) and polymerase chain reaction (pcr). results: two species, b. abortus and b. melitensis were present separately or in the same sample by spat while only separately by pcr. out of 200 samples of humans 10% and 7.5% were positive by using spat and pcr, respectively. the prevalence of the disease in males were, 12% by spat while 9% by pcr, in female, 8% by spat (males to females’ ratio of 3:2) while 6% by pcr(males to females’ ratio of 4:3). in different age groups of humans, i.e., 1-20, 21-40, 41-60, and >60 were as 10.38, 9.52, 12.00, and 7.14% by spat while 7.79, 8.33, 4.00, and 7.14% by pcr, respectively. in professional groups of humans, i.e., farmers, animal keepers, servants, house-wives and others, the prevalence was 11.42, 12.5, 0, 8.75 and 10.71% by spat while 8.57, 6.25, 0, 7.5, 7.14 and 3.75% by pcr, respectively. conclusion: the sero-prevalence of brucellosis in humans is existed in the study area.recommendation: an effective control programme of the disease is recommended.