detection of co-infection of notocactus leninghausii f. cristatus with six virus species in south korea

Co-infection with two virus species was previously reported in some cactus plants. here, we showed that notocactus leninghausii f. cristatus can be co-infected with six different viruses: cactus mild mottle virus (cmmov)-nl, cactus virus x (cvx)-nl, pitaya virus x (pivx)-nl, rattail cactus necrosis-associated virus (rcnav)-nl, schlumbergera virus x (schvx)-nl, and zygocactus virus x (zyvx)-nl. the coat protein sequences of these viruses were compared with those of previously reported viruses. cmmov-nl, cvx-nl, pivx-nl, rcnav-nl, schvx-nl, and zyvx-nl showed the greatest nucleotide sequence homology to cmmov-kr (99.8% identity, genbank accession nc_011803), cvx-jeju (77.5% identity, genbank accession lc12841), pivx-p37 (98.4% identity, genbank accession nc_024458), rcnav (99.4% identity, genbank accession nc_016442), schvx-k11 (95.7% identity, genbank accession nc_011659), and zyvx-b1 (97.9% identity, genbank accession nc_006059), respectively. this study is the first report of co-infection with six virus species in n. leninghausii f. cristatus in south korea.