|
|
|
|
effect of vkorc1 gene polymorphism on warfarin response in razavi khorasan province cardiovascular patients
|
|
|
|
|
|
|
|
نویسنده
|
zafari tahmine ,ajilian narges ,mansouri atena ,sadr-nabavi ariane ,esmaeili alireza ,jafarzadeh esfehani reza ,abtahi saeed
|
|
منبع
|
international journal of medical laboratory - 2020 - دوره : 7 - شماره : 4 - صفحه:251 -257
|
|
چکیده
|
Background and aims: warfarin is an anticoagulant agent used for many years in treating various clinical conditions such as thromboembolisms in cardiovascular disease. some patients require different doses of warfarin to reach the therapeutic international normalized ratio ratio. these patients have specific demographic characteristics. genetic polymorphisms in specific genes have been reported to be an essential factor in response to warfarin. the present study investigated the effect of these polymorphisms of genes on warfarin dose necessities in pediatric of vcorc1 gene in patients. material and methods: ninety-five patients with cardiovascular disease, who were receiving warfarin for at least three months, enrolled in the present cross-sectional study. their genomic dna was extracted from their peripheral blood, and the vkorc1 (rs9923231) polymorphism was evaluated by polymerase chain reaction and sequencing. results: among the study population, 48 patients (50.5%) had tc genotype and, 21 (22.1%) and 9 (9.5%) patients have tt and cc genotype, respectively. there was no significant relation between warfarin dose and vcorc1 genotype in our population (p<0.05). conclusions: the vkorc1 polymorphism (rs9923231) did not significantly affect the warfarin required for cardiovascular disease patients. further studies evaluating other genes such as cyp2c9 polymorphisms in our population are warranted.
|
|
کلیدواژه
|
cardiovascular disease ,genotype ,polymorphism ,vkorc1 ,warfarin
|
|
آدرس
|
islamic azad university, mashhad medical sciences branch, department of pediatric cardiology, iran, islamic azad university, mashhad medical sciences branch, department of pediatric cardiology, iran, islamic azad university, mashhad branch, innovated medical research center, department of immunology, iran. mashhad university of medical sciences, nanotechnology research center, pharmaceutical technology institute, iran, mashhad university of medical sciences, medical genetics research center, basic medical sciences institute, iranian academic center for education, culture and research, (acecr), faculty of medicine, department of medical genetics, iran, mashhad university of medical sciences, immunology research center, faculty of medicine, department of immunology, iran, mashhad university of medical sciences, medical genetics research center, basic medical sciences institute, iranian academic center for education, culture and research, (acecr),faculty of medicine, department of medical genetics, iran, islamic azad university, mashhad medical sciences branch, department of pediatric cardiology, iran
|
|
پست الکترونیکی
|
sabtahimd@yahoo.com
|
|
|
|
|
|
|
|
|
|
|
|
|
|
جهش های ژن DNMT3A در لوسمی میلوئیدی حاد و لوسمی لنفوئیدی حاد
|
|
|
|
|
Authors
|
|
|
Abstract
|
زمینه و اهداف:DNA متیل ترانسفراز3A برای تنظیم بیان ژن ضروری است و جهش ها در ژن DNMT3A در بسیاری از سرطان های خون گزارش شده است.جهش های DNMT3A در طول پیشرفت سرطان موجود است و سبب پیش آگهی منفی در بسیاری از سرطان ها می شود.بنابراین،این ژن می تواند هدفی برای درمان های جدید باشد.این مطالعه با هدف بررسی توزیع جهش های DNMT3A در بیماران لوسمی حاد ایرانی انجام شد.مواد و روش ها:در این مطالعه نمونه های 45 بیمار لوسمی حاد de novo ، شامل 23 بیمار AML و 22 بیمار ALL از فروردین 1396 تا اسفند 1396 جهت شیوع جهش های DNMT3A با PCR و سکانس مستقیم بررسی شد.نتایج:در کل 2(9.1%) مورد AML و یک(4.34%) مورد ALL دارای جهش R882H بودند .22.7% و 21.7% بیماران AML وALL به ترتیب دارای پلی مورفیسم rs368516543 بودند.جهش های DNMT3A بطور قابل توجهی با سن بالاتر در بیماران AML مرتبط بود.نتیجه گیری:این یافته ها پیشنهاد می کند که جهش های DNMT3A احتمالا بیومارکری جدید در بررسی اولیه و درمان لوسمی حاد است،هرچند مطالعات بیشتر نیاز است.
|
|
Keywords
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|