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Two-Pore K+ channel TREK-1 regulates sinoatrial node membrane excitability
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نویسنده
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unudurthi s.d. ,wu x. ,qian l. ,amari f. ,onal b. ,li n. ,makara m.a. ,smith s.a. ,snyder j. ,fedorov v.v. ,coppola v. ,anderson m.e. ,mohler p.j. ,hund t.j.
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منبع
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journal of the american heart association - 2016 - دوره : 5 - شماره : 4
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چکیده
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Background-two-pore k+ channels have emerged as potential targets to selectively regulate cardiac cell membrane excitability; however,lack of specific inhibitors and relevant animal models has impeded the effort to understand the role of 2-pore k+ channels in the heart and their potential as a therapeutic target. the objective of this study was to determine the role of mechanosensitive 2-pore k+ channel family member trek-1 in control of cardiac excitability. methods and results-cardiac-specific trek-1-deficient mice (αmhc-kcnkf/f) were generated and found to have a prevalent sinoatrial phenotype characterized by bradycardia with frequent episodes of sinus pause following stress. action potential measurements from isolated αmhc-kcnk2f/f sinoatrial node cells demonstrated decreased background k+ current and abnormal sinoatrial cell membrane excitability. to identify novel pathways for regulating trek-1 activity and sinoatrial node excitability,mice expressing a truncated allele of the trek-1-associated cytoskeletal protein βiv-spectrin (qv4j mice) were analyzed and found to display defects in cell electrophysiology as well as loss of normal trek-1 membrane localization. finally,the βiv-spectrin/trek-1 complex was found to be downregulated in the right atrium from a canine model of sinoatrial node dysfunction and in human cardiac disease. conclusions-these findings identify a trek-1-dependent pathway essential for normal sinoatrial node cell excitability that serves as a potential target for selectively regulating sinoatrial node cell function. © 2016 the authors.
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کلیدواژه
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Automaticity; K channel; Sinoatrial node; Spectrin; TREK-1
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آدرس
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dorothy m. davis heart and lung research institute,the ohio state university wexner medical center,columbus,oh,united states,department of biomedical engineering,college of engineering,the ohio state university,columbus,oh, United States, dorothy m. davis heart and lung research institute,the ohio state university wexner medical center,columbus,oh,united states,department of biomedical engineering,college of engineering,the ohio state university,columbus,oh, United States, dorothy m. davis heart and lung research institute,the ohio state university wexner medical center,columbus,oh,united states,department of biomedical engineering,college of engineering,the ohio state university,columbus,oh, United States, molecular virology,immunology and medical genetics,the ohio state university wexner medical center,columbus,oh, United States, dorothy m. davis heart and lung research institute,the ohio state university wexner medical center,columbus,oh,united states,department of biomedical engineering,college of engineering,the ohio state university,columbus,oh, United States, dorothy m. davis heart and lung research institute,departments of physiology and cell biology,the ohio state university wexner medical center,columbus,oh, United States, dorothy m. davis heart and lung research institute,the ohio state university wexner medical center,columbus,oh,united states,dorothy m. davis heart and lung research institute,departments of physiology and cell biology,the ohio state university wexner medical center,columbus,oh, United States, dorothy m. davis heart and lung research institute,the ohio state university wexner medical center,columbus,oh,united states,molecular virology,immunology and medical genetics and internal medicine,the ohio state university wexner medical center,columbus,oh, United States, dorothy m. davis heart and lung research institute,the ohio state university wexner medical center,columbus,oh,united states,department of biomedical engineering,college of engineering,the ohio state university,columbus,oh, United States, dorothy m. davis heart and lung research institute,the ohio state university wexner medical center,columbus,oh,united states,dorothy m. davis heart and lung research institute,departments of physiology and cell biology,the ohio state university wexner medical center,columbus,oh, United States, molecular virology,immunology and medical genetics,the ohio state university wexner medical center,columbus,oh, United States, dorothy m. davis heart and lung research institute,the ohio state university wexner medical center,columbus,oh,united states,department of medicine,the johns hopkins university school of medicine,baltimore,md, United States, dorothy m. davis heart and lung research institute,the ohio state university wexner medical center,columbus,oh,united states,dorothy m. davis heart and lung research institute,departments of physiology and cell biology,the ohio state university wexner medical center,columbus,oh,united states,molecular virology,immunology and medical genetics and internal medicine,the ohio state university wexner medical center,columbus,oh, United States, dorothy m. davis heart and lung research institute,the ohio state university wexner medical center,columbus,oh,united states,molecular virology,immunology and medical genetics and internal medicine,the ohio state university wexner medical center,columbus,oh,united states,department of biomedical engineering,college of engineering,the ohio state university,columbus,oh, United States
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Authors
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