Neovascularization potential of blood outgrowth endothelial cells from patients with stable ischemic heart failure is preserved

Background-blood outgrowth endothelial cells (boecs) mediate therapeutic neovascularization in experimental models,but outgrowth characteristics and functionality of boecs from patients with ischemic cardiomyopathy (icmp) are unknown. we compared outgrowth efficiency and in vitro and in vivo functionality of boecs derived from icmp with boecs from age-matched (acon) and healthy young (con) controls. methods and results-we isolated 3.6±0.6 boec colonies/100x106 mononuclear cells (mncs) from 60-ml blood samples of icmp patients (n=45; age: 66±1 years; lvef: 31±2%) versus 3.5±0.9 colonies/100x106 mncs in acon (n=32; age: 60±1 years) and 2.6±0.4 colonies/100x106 mncs in con (n=55; age: 34±1 years),p=0.29. endothelial lineage (vegfr2+/cd31+/cd146+) and progenitor (cd34+/cd133-) marker expression was comparable in icmp and con. growth kinetics were similar between groups (p=0.38) and not affected by left ventricular systolic dysfunction,maladaptive remodeling,or presence of cardiovascular risk factors in icmp patients. in vitro neovascularization potential,assessed by network remodeling on matrigel and three-dimensional spheroid sprouting,did not differ in icmp from (a)con. secretome analysis showed a marked proangiogenic profile,with highest release of angiopoietin-2 (1.4±0.3x105 pg/106 icmp-boecs) and placental growth factor (5.8±1.5x103 pg/106 icmp boecs),independent of age or ischemic disease. senescence-associated β-galactosidase staining showed comparable senescence in boecs from icmp (5.8±2.1%; n=17),acon (3.9±1.1%; n=7),and con (9.0±2.8%; n=13),p=0.19. high-resolution microcomputed tomography analysis in the ischemic hindlimb of nude mice confirmed increased arteriogenesis in the thigh region after intramuscular injections of boecs from icmp (p=0.025; n=8) and con (p=0.048; n=5) over vehicle control (n=8),both to a similar extent (p=0.831). conclusions-boecs can be successfully culture-expanded from patients with icmp. in contrast to impaired functionality of icmpderived bone marrow mncs,boecs retain a robust proangiogenic profile,both in vitro and in vivo,with therapeutic potential for targeting ischemic disease. © 2016 the authors.