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Divergent effects of miR-181 family members on myocardial function through protective cytosolic and detrimental mitochondrial microRNA targets
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نویسنده
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das s. ,kohr m. ,dunkerly-eyring b. ,lee d.i. ,bedja d. ,kent o.a. ,leung a.k.l. ,henao-mejia j. ,flavell r.a. ,steenbergen c.
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منبع
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journal of the american heart association - 2017 - دوره : 6 - شماره : 3
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چکیده
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Background-microrna (mirna) is a type of noncoding rna that can repress the expression of target genes through posttranscriptional regulation. in addition to numerous physiologic roles for mirnas,they play an important role in pathophysiologic processes affecting cardiovascular health. previously,we reported that nuclear encoded microrna (mir-181c) is present in heart mitochondria,and importantly,its overexpression affects mitochondrial function by regulating mitochondrial gene expression. methods and results-to investigate further how the mir-181 family affects the heart,we suppressed mir-181 using a mir-181- sponge containing 10 repeated complementary mir-181 seed sequences and generated a set of h9c2 cells,a cell line derived from rat myoblast,by stably expressing either a scrambled or mir-181-sponge sequence. sponge-h9c2 cells showed a decrease in reactive oxygen species production and reduced basal mitochondrial respiration and protection against doxorubicin-induced oxidative stress. we also found that mir-181a/b targets phosphatase and tensin homolog (pten),and the sponge-expressing stable cells had increased pten activity and decreased pi3k signaling. in addition,we have used mir-181a/b-/- and mir-181c/ d-/- knockout mice and subjected them to ischemia-reperfusion injury. our results suggest divergent effects of different mir-181 family members: mir-181a/b targets pten in the cytosol,resulting in an increase in infarct size in mir-181a/b-/- mice due to increased pten signaling,whereas mir-181c targets mt-cox1 in the mitochondria,resulting in decreased infarct size in mir-181c/d-/- mice. conclusions-the mir-181 family alters the myocardial response to oxidative stress,notably with detrimental effects by targeting mt-cox1 (mir-181c) or with protection by targeting pten (mir-181a/b). © 2017 the authors.
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کلیدواژه
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MicroRNA; MiR-181; Mitochondria; Mitochondrial miRNA; Mitochondrial respiratory complex IV; Mt-COX1; Oxidative stress; Phosphatase and tensin homolog; PI3 kinase; Reperfusion injury
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آدرس
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department of pathology,johns hopkins school of medicine,baltimore,md, United States, department of environmental health and engineering,bloomberg school of public health,johns hopkins university,baltimore,md, United States, department of pathology,johns hopkins school of medicine,baltimore,md, United States, department of cardiology,johns hopkins school of medicine,baltimore,md, United States, department of cardiology,johns hopkins school of medicine,baltimore,md,united states,faculty of medicine and health sciences,macquarie university,sydney, Australia, princess margaret cancer centre,university of torontoon, Canada, department of biochemistry and molecular biology,bloomberg school of public health,johns hopkins university,baltimore,md, United States, department of pathology and laboratory medicine,perelman school of medicine,university of pennsylvania,philadelphia,pa,united states,department of division of transplant immunology,department of pathology and laboratory medicine,children's hospital of philadelphia,university of pennsylvania,philadelphia,pa,united states,institute for immunology,perelman school of medicine,philadelphia,pa, United States, department of immunobiology,yale university school of medicine,new haven,ct, United States, department of pathology,johns hopkins school of medicine,baltimore,md, United States
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Authors
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