analytical methods for the determination of aspirin in its combinations with clopidogrel and some statins in medications for treatment of acute coronary syndromes (acs)

The combination of aspirin (asp) and clopidogrel (cld) has demonstrated efficacy in managing coronary syndromes, including unstable angina and myocardial infarction. this regimen prevents clotting and effectively reduces the risk of vascular events by inhibiting both adenosine diphosphate and the cyclooxygenase pathway. furthermore, cholesterol-lowering agents such as statins are also employed as a preventive measure for patients with acute coronary syndromes (acs). this article reviews the current analytical methods for the quantitative determination of aspirin in combination with clopidogrel, or the combination of the two drugs with one of the statins, in various marketed formulations in the period of 2005 -2024. the most commonly used methods for determining these combinations include chromatographic techniques such as high-pressure liquid chromatography (hplc) and thin-layer chromatography (tlc), as well as spectrophotometric methods. recent trends in the analysis of these combination samples show a preference for hplc (60%), thin-layer chromatography (tlc) (22.5%) and spectrophotometric methods (17.5%) reflecting a general shift towards more sensitive methods with higher resolution capabilities. these methods also offer the advantages of requiring smaller quantities of samples and reagents and shorter analysis times.