Preliminary study for the preparation of fatty acid bioconjugated L-asparaginase micellar nanocarrier as a delivery system for peptide anti-cancer agents

In the present study, the mixing behavior of different surfactants and fatty acid bioconjugated l-asparaginase was investigated. the amphiphilic macromolecules were achieved by covalent linkage of fatty acids with different chain lengths (c12, c16, and c22) to the native enzyme, l-asparaginase. the amino group of l-asparaginase lysine residue was conjugated to the carboxylic group of fatty acids, using a carbodiimide activator. the particle sizes of resulted micellar nanocarrier before and after lyophilization and enzyme activity was investigated. among all surfactants, pluronic f-127 with fatty acid bioconjugated l-asparaginase presented more plasma and pbs half-life and higher activity value after lyophilization. these findings from l-asparaginase modification by fatty acid and surfactants indicate a promising stabilized product that may serve as a new candidate for medical purposes.