Concurrent analysis of Simvastatin and citicoline using a Reversed-phase High Performance Liquid Chromatography-Ultra Violet Method

To develop and evaluate a sensitive, accurate, rapid and reproducible high performance liquid chromatography analytical method for concurrent assay of simvastatin, a hyperlipidemia controlling agent, and citicoline, a psychostimulant agent, a c18 column (eurosphar 100-5, 150 mm ×4.6 mm) used as a reversed stationary phase and mobile phase was water (previously adjusted with phosphoric acid to a ph of 5.5), methanol and acetonitrile (20:20:60) with the flow rate 1.0 ml/min. the ultraviolet detector was set at 247 nm. a linear correlation between each concentration and its own auc within concentration ranges of 15 to 100 μg/ml for citicoline and 7.5 to 50 μg/ml for simvastatin with a correlation coefficient 0.9969 for citicoline and 0.994 for simvastatin were produced. the within and between-day precision and accuracy were both in acceptable ranges. the outcomes of these tests show an accurate, rapid and robust hplc-uv method for successful analysis of both simvastatin and citicoline simultaneously.