A reversed-phase high performance liquid chromatography approach for analysis of 5-Fluorouracil

A reproducible, sensitive, accurate and selective high performance liquid chromatographic (hplc) method with ultraviolet absorbance detection has been developed and validated to quantitate 5- fluorouracil (5-fu), an antimetabolite chemotherapeutic agent used in treatment of colorectal cancer as a drug of choice. chromatographic separations were performed on a c18 column (eurosphar 100-5, 150 mm × 4.6 mm) as the reversed stationary phase, eluted with a mobile phase composed of deionized water and methanol (95:5) with the flow rate of 1.0 ml/min. the uv wavelength was set at 261nm. the method produced linear responses throughout 5-fluorouracil range of 25-500 ng/ml with a correlation coefficient of 0.998. a limit of quantitation (loq) was established at 25 ng/ml. the within-day and between-day precision and accuracy were both in acceptable ranges. the outcomes of these tests indicate a proper separation efficacy as well as accuracy and sensivity of method which is a potential tool in many pharmaceutical studies such as drug delivery of this anti-cancer agent.