Production of a novel multi-epitope vaccine based on outer membrane proteins of Klebsiella pneumoniae

Klebsiella pneumoniae is a hospital-acquired pathogen that leads to various infections. hence, efforts to develop an effective vaccine against that pathogen are well documented. our interest is the production of the previously designed multi-epitope vaccine construct against the k. pneumoniae in a prokaryotic host. therefore, a new construct containing the nucleotide sequence of the novel vaccine was successfully expressed in escherichia coli and then purified by ni-nta spin column. the purified recombinant protein can be considered as potential vaccine candidate for wet-laboratory analysis aiming to fight k pneumoniae.