The influence of kapok (Ceiba pentandra) seed oil supplementation on cellulolytic enzyme and rumen microbial fermentation activity of local sheep

This research was conducted to study the influence of kapok seed oil (kso) supplementation on cellulolytic enzyme and microbial fermentation activity. sheep rumen fluid was used as enzyme source and inoculant,whereas carboxymethylcellulose (cmc) was used as the substrate. there were 4 levels of kso supplementation as treatment,i.e. : 0% (t0),5% (t1),10% (t2),and 15% (t3). two measured variables were reduced sugar production rate and gas fermentation production. the data were analyzed by analysis of variance in completely randomized design. the result showed that reduced sugar production rate in t0,t1,t2 and t3 treatment groups were 2.58; 2.93; 2.08 and 1.58 mg/g cmc/minute,respectively,whereas gas production were : 15.97; 13.26; 10.54 and 7.57 mg/g cmc,respectively. kapok seed oil supplementation up to 5% dm of cellulose substrate (cmc) did not influence the ruminal cellulolytic enzyme activity. the kso supplementation level 10% - 15% decreased the ruminal cellulolytic enzyme activity. © 2010 diponegoro university. all rights reserved.