The surface character of staphylococcus aureus isolated from subclinical mastitis of dairy cow supporting adherence to udder epithelial cell

Staphylococcus aureus (s. aureus) is one among pathogen bacteria that caused mastitis in dairy cow in indonesia. its ability to initiate infection depends on the surface character of the bacteria. the surface character of s. aureus is determined by the corelation of red blood cell hemagglutination activity,hydrophobicity,production of protein a,and bacterial adherence to the mammary epithelial cells of the host. this research was designed to characterize the surface of s.aureus,which correlates to bacterial adherence. four isolates of s. aureus were taken from subclinical mastitis milk of dairy farm in malang,east java. the surface character of s.aureus was determined by performing the red blood cell hemagglutination test using the dairy cow erythrocyte suspension with concentration of 0.5%,1%,and 2%. the hydrophobicity expression was determined by salt aggregation test (sat) method using the ammonium sulfate suspension with concentration of 1.2 m,1.6 m,2.0 m,2.4m,and 3.2 m. serum soft agar (ssa) test was used to indicate the production of protein a; ability of adhesion was done by adhering the s.aureus,which were already labelled by fluorescent ichtiocyanate (fitc) to the mammary epithelial cell of post-calving mice. result of this this research showed that the surface characters of s.aureus,which correlates to the adherence,comprise of erythrocytes hemagglutination ability,hydrophobicity,and protein a production. isolates of s.aureus,which is hydrophobic,possess haemagglutinine,and protein a were able to adhere to mammary epithelial cell with higher quantity. © 2010 diponegoro university. all rights reserved.