Histological study on the pancreatic β-cell number of indigenous chicks in first crossbred (F1)

The objective of this study was to evaluate the total pancreatic β-cell number of chicks in first crossbred (f1) with potential as broiler. the total number of twenty day-old male chicks consisted of docs cobb 500 broiler line as a control group and those of pelung and the crossbred of pelung and cemani chicks with the average body weight of 30 gram as materials. they were divided into four groups,in which each group has 5 male doc's. group 1 was docs from parent of cobb 500 broiler as the control; group 2 was docs from parent of pelung; group 3 was crossbred docs from male parent cemani line and female parent pelung line; and group 4 was crossbred docs taken from male parent of pelung and female parent of cemani. chick body weight of each group was measured at 7th week. histological process was carried out on five docs of each group previously raised until seven weeks old and at the end of experiment all chicks were euthanized for pancreatic organs,particularly tissue samples of pancreas,made as histological preparates. data from the histological probe on total pancreatic β-cell number was statistically analyzed using the anova of one way classification,and then followed by the lsd-test. result of the study showed that average body weights of docs in the control group of cobb 500 (2770±58.31) significantly higher (p<0.05) compared to the groups of 2,3,and 4 (652.4±32.77,570.4±14.45,and 628±37.2 g,respectively). total pancreatic β-cell number in the control group of cobb 500 broiler chicks (2.76x106±0.20x 106) significantly higher compared to the groups of 2,3,and 4 (1.93 x106±0.34 x106,1.64x106±0.30x106,and 1.71 x106±0.19 x106,respectively). the results of this study revealed that total pancreatic β-cell number can be used as an indicator of the growth and development of chick. © 2010 diponegoro university. all rights reserved.