Identification of melanocortin 1 receptor (mc1r) gene based on coat color of bali cows of kupang by usingthe PCR-rflp method

The objective of this study was to identify the melanocortin 1 receptor (mc1r) gene in sorrel-,black- and white-bali cows originated from kupang-east nusa tenggara. blood samples were taken from 46 cows consisted of of 17,18 and 8 of sorrel,black and 8 heads,respectively,and 3 heads of bull. the pcr-rflp method was performed to identify the gene. amplification of pcr gene mc1r of kupang's bali cattle (kbc) was 296 bp. the digestion by using mspi enzyme showed that there were ee genotype (169,136 bp) in bali bull,sorrel and white cows. two 2 genotypes,those were ee (169,136 bp) and ee (296,169,136 bp) were found in black bali cow. the frequency of e alele of kbc was 0.99 (99%) and e alele was 0.01 (1%). mc1r gene in kbc was monomorphic and the it can be used as the marker of the coat color in the population. © 2013 diponegoro university. all rights reserved.