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The hypo-osmotic swelling test in fresh garut ram spermatozoa
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نویسنده
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nalley w.m.m. ,arifiantini r.i.
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منبع
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journal of the indonesian tropical animal agriculture - 2013 - دوره : 38 - شماره : 4 - صفحه:212 -216
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چکیده
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The study was aimed to determine the optimal time to evaluate the maximum numbers of swollen sperm after expose to hypo-osmotic solution for testing membrane integrity in fresh garut ram semen. a total of 24 ejaculate samples from eight sexually mature garut ram were collected using artificial vagina. after macroscopic evaluation,10 μl undiluted semen was gently mixed in each of the 2 ml hypoosmotic solution and incubated at 37oc for 1 hour. two hundred of coil and non-coiled sperm were observed from five different fields every 15 minutes. results demonstrated that the maximum percentage of coiling sperm positive to hos test was at 30 minutes and no differences among 8 garut rams used. © 2013 diponegoro university. all rights reserved.
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کلیدواژه
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Garut rams semen; Hypo-osmotic swelling test; Membrane integrity
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آدرس
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faculty of animal science,nusa cendana university,jln. adi sucipto penfui kupang,east nusa tenggara, Indonesia, faculty of veterinary medicine,bogor agricultural university,darmaga campus,bogor 16680, Indonesia
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Authors
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