Purification of angiotensin converting enzyme inhibitory peptide derived from kacang goat meat protein hydrolysate

The objective of this study was to identify the angiotensin converting enzyme (ace) inhibitory peptide derived from kacang goat meat protein hydrolysate. kacang goat meat loin section was hydrolyzed with pepsin,trypsin and chymotrypsin. protein hydrolysate of kacang goat meat was then tested the protein concentration and ace inhibitory activity. ace inhibitory peptide of the protein hydrolysate was purified through several steps of purification by column sep-pak plus c18 cartridge and rp-hplc using a cosmosil column 5pe-sm,4.6 x 250 mm. the sequence of amino acid of ace inhibitory peptide was identified by amino acid sequencer. the results showed that amino acids sequence of ace inhibitory peptide derived from protein hydrolysate of kacang goat meat was leu-thrglu- ala-pro-leu-asn-pro-lys-ala-arg- asn-glu-lys. it had a molecular weight (mw) of 1581 and occurred at the position of 20th to 33rd residues of b-actin of goat meat protein (capra hircus). the ace inhibitory activity (ic50) of the peptide was 190 mg/ml or 120 mm. © 2013 diponegoro university. all rights reserved.