Genetic diversity of Kejobong goat based on mitochondrial DNA D-loop sequence

This study was aimed to find out the diversity of mtdna d-loop at kejobong goat. the complete mtdna d-loop sequence of 12 goat blood samples were analyzed from 4 different location in purbalingga regency,central java province,sub-districts kejobong,pangadegan,bukateja,and kaligondang. the mtdna d-loop was extracted from blood sample. dna obtained were amplified by pcr (polymerase chain reaction) method using primers (5’-tcactatcagcacccaaagc-3’) as forward and (5’-ggcattttcagtgccttgct-3’) as reverse and subsequently sequenced. after nucleotide sequencing analysis conducted,548 bp along was obtained. nucleotides were then aligned with capra hircus (genbank access no.: kf952601.1) and apparently there were 11 different sites on the segment of mtdna d-loop. five sites could be used as a specific marker to distinguish between the capra hircus and kejobong goat,namely at the site of 317 (a-g),403 (t-c),434 (t-c),537 (c-t),and 553 (a-g). nucleotide sequence analysis also contained seven different haplotypes. it was concluded that the distribution of the different sites showed different haplotype patterns in kejobong goat. © 2014 diponegoro university. all rights reserved.