Pre-slaughter stress estimation by fourier transform infrared spectroscopy analysis

The objective of the study was to analyze the potential of fourier transform infrared (ftir) as stress estimation instrument through measurement of catecholamine (ca) and cortisol (co) levels by invasive and non-invasive methods. twelve heads of ongole grade (po) cow from slaughterhouse were used in this study. twenty four urine samples of pre-slaughter and slaughtering were collected to evaluate the ca and co levels by elisa method and fourier transform infrared (ftir). data of ca and co levels were statistically analyzed to determine the difference between pre slaughter and slaughter conditions. ftir spectra were analyzed using chemo-metrics software. the results showed that the levels of urinary cortisol were 2.12±1.68 ng/dl of pre-slaughter and 7.58±3.89 ng/dl of slaughtered and the levels of urinary catecholamine in pre slaughter and slaughter were 3.07±2.05 and 4.15±2.68 ng/dl respectively. the results showed a correlation between the ftir spectra and the results of elisa analysis. ftir spectrums were distributed in different quadrants,this was caused by differences of ca and co levels between pre-slaughter and slaughtered. it can be concluded that ftir can be supposed to analyze the status of stress in animals,especially in cattle. © 2014 diponegoro university. all rights reserved.