Single nucleotide polymorphisms of lipoprotein lipase gene and its association with marbling quality in local sheeps

Lipoprotein lipase (lpl) is a key enzyme that plays in metabolism and transport lipoprotein and therefore has an influence on blood triglyceride levels. lpl controls triacylglycerol partitioning between adipose tissue and muscle that increases fat storage or provides energy in the form of fatty acids for muscle growth. the research was aimed to explore single nucleotide polymorphisms of lpl gene and to associate snp with marbling quality. a total of 66 genomic dnas consisted of sumatera thin-tail ed sheep (50 heads) and garut sheep (16 heads) were used in this study. polymerase chain reaction was used to amplify genomic dna and direct sequencing method was to identify polymorphism sequences. the sequences were analyzed with bio edit and mega 5.2. the blast sequence was obtained from gene bank x.68308.1. the association between the genotype and marbling quality was analyze by one way anova and further between mean differences were tested using least sgnificant difference. the results showed that 3 novel snps i.e. insertion g.26>c; insertion g.27> g and c.192t>c on garut sheep and a snp insertion g.26>c/g on sumatera thin-tail ed sheep. the diversity of lpl gene at c.192t>c was associated with heneicosanoic acid,whereas tt genotype (0.04%) was higher than cc (0.03%) and ct (0.02%). © 2015 diponegoro university. all rights reserved.