Detection of oxytetracycline in broiler chicken meat marketed in several cities in Java island using enzyme-linked immunosorbent assay (elisa) method

Oxytetracycline (otc) is one of the tetracycline (tcs) broad-spectrum antibiotics widely used in the chicken industry. however,improper use of otc with excessive doses potentially leads to residue formation in animal products that can be harmful to consumers in the form of allergic reaction or resistance. this study aimed to detect otc residues in broiler chicken meat,marketed in traditional markets and supermarkets in depok,bekasi,bandung,cilegon,surakarta and yogyakarta using indirect competitive enzyme-linked immunosorbent assay (icelisa) method. the analyses of 67 broiler meat samples showed only 1 (1.5%) sample was positive for the otc residue at 86.1 ng/g which meant below the maximum residue limits of permissible otc (100 ng/g). nevertheless,a stricter regulation for the use of otc in the poultry industry and the monitoring of its residue in chicken products prior to marketing is still necessary to avoid the adverse effects of the residue present in animal products. © 2015 diponegoro university. all rights reserved.