oleuropein reduces cisplatin-induced nephrotoxicity in human embryonic renal epithelial “gp-293” cells

Background and aim:the protective effect of oleuropein,a chief polyphenol compound in olive tree, on cisplatin-induced nephrotoxicity in human cultured renal tubular cells, gp-293, was investigated. materials and methods:cell viability was determined by the mtt assay. cleaved caspase-3 and bax: bcl2 ratioas biochemical parameters of cellular apoptosis, was assessed using western blot analysis. results:our data showed that cisplatin in the dose of 55μg/ml significantly can reduced cell viability, increased caspase-3 activation and bax: bcl2 ratio in gp-293 cells after 24hr. incubation of the cisplatin treated cells, with 20μg/ml oleuropein decreased the cisplatin-induced cell toxicity, as well as prevented caspase-3 activation and reduced bax: bcl2 elevation ratio. conclusions: oleuropein protects against cisplatin-induced cellular toxicity. this could be associated with the prevention of cellular apoptosis.