Determination of Astaxanthin in Trout by High Performance Liquid Chromatography Coupled with Visible Detection

Background: astaxanthin is widely and naturally distributed in marine animals including, crustaceans, such as shrimp, crabs, and fish, such as salmon and trout. methods: a total of 20 trout samples were collected from local market and were analyzed for astaxanthin via hplc-uv. analyte extraction was done by water, methanol, dichloromethane, and acetonitrile 4.5:85:5:45.5, v/v/v/v. results: despite the optimal conditions, analytical property of the method containing lower limit of quantitation (2.00 ng g-1) and upper limit of quantitation (1000 ng g-1), linearity (r2=0.992), and recovery (103%) were acceptable. the results showed the range of 105 ± 0.09 - 875 ± 0.020 ng g-1. conclusion: the method was successfully used to quantify the target analytes in all trout samples.