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optimization of mobile phase of high performance liquid chromatography using full factorial design for simultaneous estimation of thiocolchicoside and dexketoprofen trometamol in tablets
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نویسنده
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trivedi shivani a. ,joshi dharati m. ,patel samir g. ,patel rajendra k. ,patel archita j.
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منبع
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iranian journal of analytical chemistry - 2015 - دوره : 2 - شماره : 1 - صفحه:14 -21
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چکیده
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The present study was aimed to develop and validate a high performace liquid chromatography method for determination of thiocolchicoside and dexketoprofen trometamol in combined dosage form. the proposed hplc method utilizes an agilent eclipse c-8 column (5 µm, 250×4.6 mm) at ambient temperature. a 23 full factorial design was performed for optimization of mobile phase for separation of two drugs. for the factorial design, the dependent variables chosen were % of acetonitrile, % of 0.1% o-phosphoric acid and ph. the optimum mobile phase consisted of acetonitrile: 0.1% o-phosphoric acid in water (41.9:58.1; ph 2.6). the flow rate was kept 1 ml/min with uv detection at 254 nm. the run time was found to be 1.41 min and 7.58 min for thiocolchicoside and dexketoprofen trometamol, respectively. the method was validated as per ich guidelines. the calibration plots were linear in the range of 1-5 μg/ml with r2=0.998 for thiocolchicoside and 6-30 μg/ml with r2 = 0.995 for dexketoprofen trometamol. the % recovery values were found to be 98.52 ± 1.21 and 98.16 ± 1.45 for thiocolchicoside and dexketoprofen trometamol, respectively. the minimum detectable and minimum quantifiable amounts were found to be 0.11 and 0.35 μg/ml, respectively for thiocolchicoside and 1.41 and 4.38 μg/ml, respectively for dexketoprofen trometamol. the developed method could be utilized successfully in simultaneously analyzing the drugs in bulk as well as combined dosage form.
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کلیدواژه
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thiocolchicoside ,dexketoprofen trometamol ,high performance liquid chromatography ,full factorial design
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آدرس
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k. b. institute of pharmaceutical education and research, india, k. b. institute of pharmaceutical education and research, india, charusat university, ramanbhai patel college of pharmacy, india, k. b. institute of pharmaceutical education and research, india, k. b. institute of pharmaceutical education and research, india
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پست الکترونیکی
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architajpatel@gmail.com
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بهینه سازی فاز متحرک کروماتوگرافی مایع با کارآیی بالا با استفاده از طراحی فاکتوریال کامل برای ارزیابی همزمان یتوکلوچیکوساید
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Authors
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ترای ودی شیوانی آ. ,جوشی داراتی ام. ,پاتل سمیرجی. ,پاتل راژندرا کا. ,پاتل آرشیتا جی.
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Abstract
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هدف مطالعه حاضر عبارت بود از توسعه یک روش کروماتوگرافی با کارآیی بالا برای تعیین یتوکلوچیکوساید (TC) و دکستپروفن ترومتامول (DKT) در نمونه های قرص مکملی این داروها. بعد از بهینه سازی نهایی حد تشخیص برای TC و DKT به ترتیب 11/0 و 35/0 میکروگرم بر میلی لیتر بدست آمد. برای بهینه سازی پارامترها از روش طراحی فاکتوریال کامل (FFD) استفاده شد.
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Keywords
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