simultaneous determination of atenolol and propranolol in pharmaceutical formulations by third derivative spectrophotometric method

In this study, a third derivative spectrophotometric method based on zero-crossing technique is developed for the simultaneous determination of atenolol and propranolol. effective parameters including temperature and ph were investigated and optimized. under optimum experimental conditions (temperature: 10 oc and ph: 8-10), calibration curves were linear between concentration ranges of 0.8 to 12.9 μg ml-1 of atenolol and 0.3 to 6.4 μg ml-1 of propranolol. the limit of detection was 0.2 μg ml-1 and relative standard deviation was lower than 3%. the proposed method was applied for the simultaneous determination of both drugs in the pharmaceutical formulations. acceptable recoveries of the analytes, ranging from 97 to 107%, were obtained