preparation and biological activity of new collagen composites, part iii. collagen/(ag/rgo) and collagen/(ag/rgo/sio2) composites

The aim of this study is preparation, characterization, and biological activity evaluation of 2 new collagen composites, collagen/( ag/rgo) and collagen/(ag/rgo/sio2) with expected antimicrobial activity. the loading of collagen matrix by self-prepared ag/rgo and ag/rgo/sio2 was varied. the morphology of these antimicrobial agents, as well as of the corresponding collagen composites, was observed by sem. their biological activity was evaluated against 3 gram-negative and 3 gram-positive microbial strains, as well as against 3 type eukaryotic cells: osteoblast, fibroblast, and epithelial. dependent on the concentration of ag/rgo, well pronounced activity against gram-negative bacterium (e. coli), fungus (c. lusistaniae) and 2 gram-positive bacteria (s. epidermidis and b. cereus) was found for collagen/(ag/rgo) composites at weight ratios of 2:1, 2:0.8, and 2:0.6, at the last one accompanied by low cytotoxicity. dependent on the concentration of ag/rgo/sio2 biological activity was found for the collagen/(ag/rgo/sio2) composites also, better expressed than that of collagen/(ag/rgo) composites with the same level of antimicrobial agent loading. the experimental results demonstrated that the doping of rgo with ag nanoparticles increases the antimicrobial activity of the collagen/( ag/rgo) composites. improving its dispersion, the silane matrix in the ag/rgo/sio2 additionally increases the biological activity of the studied collagen/(ag/rgo/sio2) composites and their well-pronounced, broad spectrum (against 3 gram-negative, 2 gram-positive bacteria and a fungus), antimicrobial activity is similar to that of the broad spectrum antibiotics. the novel collagen/( ag/rgo) and collagen/(ag/rgo/sio2) composites are promising antimicrobial collagen biomaterials that widen the assortment of such for variety biomedical applications.