Effect of High Intensity Interval Training With Flaxseed on Interleukin-1 Beta and Lipocalin-2 Gene Expressions in the Heart Tissue of Rats

Background: inflammation plays an important role in the development of atherosclerosis. interleukin-1 beta (il-1β) and lipocalin-2 (lcn-2) are important inflammatory markers in the pathogenesis of atherosclerosis. objectives: this study aimed to investigate the interactive effects of high intensity interval exercises (hiit) with flaxseed oil consumption on il-1β and lcn-2 gene expressions in the heart tissue of rats. methods: in this experimental study, 20 adult male wistar rats were randomly selected and divided into four groups of five rats: (1) control, (2) hiit, (3) 20 mg/kg of flaxseed oil, and (4) hiit with mg/kg 20 flaxseed oil. groups 2 and 4 ran on a treadmill for 10 weeks and five weeks each, with 90% to 95% intensity of vo2max . for analysis of the research findings, kolmogorov-smirnov statistical analysis and two-way anova were used (p ≤ 0.05). results: ten weeks of hiit (p = 0.001) and flaxseed oil consumption (p = 0.03) had a significant effect on reducing the lcn-2 gene expression in the heart tissue of rats. however, 10 weeks of hiit (p = 0.19) and flaxseed oil consumption (p = 0.19) had no significant effect on il-1β gene expression in the heart tissue of rats. in addition, 10 weeks of hiit with flaxseed oil has no interactive effects on il-1β (p = 0.91) and lcn-2 (p = 0.99) in the heart tissue of rats. conclusions: although it seems that the simultaneous use of flaxseed oil with hiit does not have interactive effects on il-1β and lcn-2 gene expressions in the heart tissue of rats, the use of flaxseed oil and hiit alone results in improved lcn-2 gene expression in the heart tissue of rats.