correlation between expression of hsa-mir-490-5p and nfat5 in peripheral blood mononuclear cells obtained from breast cancer patients

Breast cancer is a complex genetic disease that has an average annual incidence of two million people and the second leading cause of death among women all over the world. micro-rnas are consistently reported to regulate gene expression in all cancers. the present study, the correlation between the expression of hsa-mir-490-5p and nuclear factor of activated t-cells 5 (nfat5) in breast cancer were investigated. nfat5 nuclear accumulation occurs regardless of wnt/β-catenin activated signaling in a substantial portion of breast cancer. the analysis of prediction target and dual-luciferase reporter assays supported that hsa-mir-490-5p directly targeted nfat5 and suppressed the expression of nfat5. in a crosssectional comparative study, peripheral blood samples were collected from 30 subjects with breast cancer and 30 healthy individuals as a control group. total mrna was extracted from peripheral blood mononuclear cells (pbmcs) and cdna was synthesized to study the nfat5 and hsa-mir- 490-5p gene expression variations by real-time pcr. a significant decrease was observed in gene expression and sera concentration of nfat5, hsamir- 490-5p in pbmcs of breast cancer patients. the obtained results indicated that hsa-mir-490-5p acts as oncomir in serum by targeting nfat5 direct (p<0.05).