association between mspi polymorphisms of the apolipoprotein a-i gene and hyperlipidemia in an iranian population

Background: one of the most important metabolic disorders is hyperlipidemia. apoai protein plays an important role in lipoprotein metabolism. in this study, the association between mspi polymorphisms in the promoter (g-75a) and first intron (c83t) of the apoai gene and hyperlipidemia was investigated in semnan, iran. methods: a total of 151 unrelated subjects were divided into two groups: the hyperlipidemic (n = 75) and control (n = 76) groups. genotyping was carried out by polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp). results: in the hyperlipidemic group, the frequency of the (+-) variant of the mspi (c83t) locus was higher than that in the control group (p < 0.05). significant differences were found in serum hdl-c and apoai levels between different genotypes of mspi (c83t) (p < 0.001 and p = 0.02, respectively). in the hyperlipidemic group, the odds ratios for the (+-) and (–) genotypes of the mspi (c83t) locus in comparison to that for the (++) genotype were 0.26 (p= 0.023) and 3.62 (p = 0.254), respectively. the allelic frequency at the (g-75a) locus was not significantly different in the hyperlipidemic and control groups (p = 0.36). the serum levels of lipid and lipoprotein were not significantly different for all genotypes of mspi (g-75a). the aa/++ and gg/– haplotypes had the lowest and highest apob/apoai ratios, respectively (0.73 ± 0.03 vs. 1.9). conclusions: the presence of (+) and a alleles in the apoai (c83t) and (g-75a) haplotypes in the semnan population may be protective against cardiovascular disease.