study of ccr5-59353c/t polymorphism in the iranian patients with chronic hepatitis b virus infection

Background: ccr5 is identified as one of the most important chemokine receptors with a major role in the creation of chemotaxis and mobilization of immunocompetent cells and moving them toward the liver for thorough cleaning of the virus. ccr5-59353 (c/t) is an important promoter polymorphism of chemokine receptor 5. some studies showed a relationship between ccr5-59353 (c/t) polymorphism and clearance or persistence of hepatitis b virus (hbv) infection. objectives: the current study aimed at developing polymorphism ccr5-59353 (c/t) in iranian patients with chronic hbv infection. methods: a total of 200 blood samples including 100 healthy controls and 100 hbsag-positive patients were randomly selected. samples were tested for hbsag by the enzyme-linked immunosorbent assay (elisa) and hbv-dna by the polymerase chain reaction (pcr) method. genomic dna was extracted from blood buffy coat using the salting out method. ccr5-59353 (c/t) polymorphism was genotyped by the allele specific amplification (asa) pcr. chi-square test was used for statistical analysis. results: five percent of control samples and 12% of patient samples had cc mutant genotype. nevertheless, there was no significant difference in genotypes frequency of ccr5-59353 between the groups (p = 0.1). conclusions: it seems that ccr5-59353 polymorphism was not associated with chronic hbv infection outcome in the iranian population. however, the frequency of cc genotype was higher in the patient group (12%) than the control group (5%).