Screening the Biotransformation Metabolites of α-Pinene by two Bacterial Strains Paenibacillus popilliae 1C, and Streptomyces rochei AB1

Introduction: biotransformation can be an effective tool for the structural modification of bioactive natural and synthetic compounds to synthesize novel and more potent compounds. the present study describes the biotransformation of α-pinene using two bacterial strains peanibacillus popilliae 1c and streptomyces rochei ab1, isolated and identified in our previous study.materials and methods: the inhibitory concentration of the target substance against both strains was evaluated as 15 mg/ml. however, for technical considerations, a concentration of the α-pinene at 10 mg/ml as biotransformation assimilable concentration (bac) was used to carry out the biotransformation process. for the biotransformation highlighting, both strains were cultivated on the rich medium (luria-bertani (lb) for peanibacillus popilliae 1c and international streptomyces project 9 (isp9) for streptomyces rochei ab1) and poor medium (minimum medium (mm) for 1c and isp9 for ab1). the chemical composition and percentage content of each compound were performed by gc/ms and gc/fid, respectively.results: the gc/ms analysis revealed that all the biotransformation products were hydrocarbon and oxygenated monoterpenes, which can be divided into two groups. the first group of 11 compounds (verbenene, isocarveol, e-2,3-epoxycarane, γ-terpinene, dehydrolinalool, α-campholene aldehyde, menthol, carvacrol, limonene dioxide, piperitenone, ocimenol) is described for the first time in the biotransformation of α-pinene and a second group bringing together 8 compounds previously reported (trans verbenol, p-cymen-8-ol, α-terpineol, myrtenol, verbenone, trans sobrerol, d-limonene, pinocarvone).conclusions: these bacterial strains possess distinctive biocatalytic capacities toward α-pinene which leads to the production of other secondary metabolites.