The Association of -1031 T/C TNF-α Gene Promoter Polymorphism with the Incident of Gastric Carcinoma Among Iraqi Patients

Introduction: among all the different types of cancer, gastric carcinoma (gc) has become the most frequently diagnosed and has continued to be a major public health issue in the last few decades worldwide. association between the polymorphisms of the (t/c) -1031 tnf-α promoter gene sequence (rs1799964) and the incidence of gastric carcinoma was tested in iraqi patients undergoing 5-fu plus cisplatin, patients without chemotherapy, and healthy controls.materials and methods: blood samples were collected from patients and control groups to carry out the molecular and immunological diagnostic tests. two ml of blood were collected in edta tubes for (t/c) -1031 tnf-α genotyping by using the rflp technique. the serum part was used for the purpose of immunological tests via elisa technique.results: findings revealed that the homozygous wild genotype (t/t) was more abundant than other genotypes (t/c and c/c) in different groups of this study (82, 73, and 72% in control, patients under treatment, and patients without treatment respectively). from the chi-square (data of the p value), there was no significant differences between genotypes in the different groups. tnf-α concentration increased significantly in heterozygous (t/c) and homozygous (t/t) genotypes in patients without treatment (p = 0.0143) and was highly significant in healthy control samples (p = 0.0003). the results showed there were non- substantial differences (p = 0.1083) in the tnf-α concentrations between different genotypes in patients treated with chemotherapy.conclusions: the genotyping study through the rflp technique and allele frequency measurement revealed that the homozygous wild genotype (t/t) was more frequent compared with the other genotypes in different groups of this study. however, tnf-α concentration significantly increased in heterozygous (t/c) genotypes. non-significant differences in tnf-α concentration were detected among different genotypes in gastric carcinoma patients under treatment of chemotherapy.