Fabrication of an amperometric glucose biosensor based on a Prussian blue/carbon nanotube/ionic liquid modified glassy carbon electrode

An amperometric glucose biosensor was developed based on synergistic contributions of prussian blue (pb) and a bucky gel (bg) consisting of carbon nanotubes (cnts) and ionic liquid (il). the pb nanoparticles were first deposited onto the surface of a bg modified glassy carbon (gc) electrode (bg/gc). then,the ni2+ ions were electrochemically inserted into the pb lattice to improve its stability at physiological ph. afterwards,glucose oxidase (gox) was immobilized on the bg/gc electrode using a cross-linking method. amperometric measurements of glucose were performed at -0.05 v vs. ag/agcl in 0.05 m phosphate buffer solution at ph 7.4. the glucose biosensor exhibited a sensitivity of 45.03 µa/(mm.cm2) with a detection limit of 5x10-7 m. the amperometric response was linear in the range of 5x10-7 to 8.3x10-4m. © 2017,baqiyatallah university of medical sciences. all rights reserved.