prevalence of mycoplasma genitalium and mycoplasma hominis isolates among women with cervicitis referred to karaj health care centers

Background: mycoplasma is a genus of bacteria often found in the normal flora of the mouth, respiratory system and urogenital tract; but potentially pathogenic species also exist which can cause serious respiratory and genital diseases in human including postpartum fever, pelvic inflammatory infections, and pyelonephritis. the aim of this study was to evaluate the prevalence of mycoplasma genitalium and mycoplasma hominis in women who referred to the health centers in karaj and investigate the susceptibility of m. genitalium strains against fluoroquinolone antibiotics. materials and methods: endocervical swabs were taken from 200 women with cervicitis. nucleic acid amplification tests (naats) were performed for detecting mgpa gene in m. genitalium and rnh gene in m. hominis. mutations in parc and gyra genes, as well as antibiotic resistance, were studied in positive samples of m. genitalium. results: m. genitalium(n=9) and m. hominis(n=11) positive samples were found among samples obtained from women with cervicitis. positive samples of m.genitalium were examined for isolating the parc and gyra genes. six sequences of these genes were analyzed by mega5 software. mutation in parc gene was observed in one sequence which %16 shows resistance. conclusion: m. hominis and m. genitalium were detected in 5.5% and 4.5% of samples, respectively. our findings showed a relatively medium prevalence of m. hominis and m. genitalium in women with cervicitis in alborz province. the sequencing results of gyra and parc genes in this study represent the occurrence of mutations which drive fluoroquinolones resistance. therefore, further studies in this field are needed in iran and other countries, and to address the problems of increasing antibiotic-resistant strains, irregular administration and antibiotic susceptibility testing, more attention should be paid.