Potential Neuroprotective Effect of Apis dorsata Honey against Morphine Tolerance: An in vivo Study

Background: to determine the effects of apis dorsata honey on the development of morphine tolerance and oxidative stress in rats.materials and methods: a total of 40 male sprague dawley rats were injected (subcutaneous) with 10 mg/kg of morphine following oral administration of a. dorsata honey (0.5, 1.5, and 2.5g/kg). on day 15, the rats were euthanized, and the thalamus, spinal cord, and hippocampus were homogenized to assess inos and mda using elisa kits.results: the honey of a. dorsata significantly prevented morphine tolerance to analgesia in the hotplate test on day 14 (p<0.05). the biochemical assessment showed that a. dorsata honey significantly reduced mda formation in the brain regions compared to the morphine control group at dose 2.5g/kg. elevation of inos caused by chronic morphine intake was reduced in a. dorsata honey co-treatment.conclusion: this study suggests the therapeutic role of a. dorsata honey in preventing morphine tolerance via inhibition of oxidative stress.