enhanced bioremediation of brass crude-oil (hydrocarbon), using cow dung and implication on microbial population

The present study has used soil samples from nigeria, contaminated with brass crudeoil, to determine its biodegradation through enhanced biostimulation with cow dung and periodic aeration. over a period of twentyeight days, the hydrocarbonutilizing bacteria (hub) and hydrocarbonutilizing fungi (huf) have been counted and identified. results from biodegradation of the brass crudeoil over the aforementioned period show that amended crudeoilspiked soil has had 54.82% degradation while for amendment and periodic turning this has been 55.90%, not significantly higher than the former at p≤0.05. also degradation of spiked soil without cow dung amendment has been 16.13%. the identified hub are staphylococcus aureus, pseudomonas aeruginosa, bacillus subtilis, and streptococcus thermophillus, with individual occurrence of 18.52% as well as proteus vulgaris and micrococcus luteus with 11.11% and 14.81% occurrence, respectively. also, the occurrence rate of huf like aspergillus flavus, a. niger, penicillium chrysogenum, trichothecium roseum, and penicillium citrinum have been 15.63% each;whilefor alternaria alternata and neurospora crazza it has been 6.25% and for saccharomyces cerevisae and a. fumigatus, 9.38%and3.13%, respectively. the study concludes that amendment with cow dung and periodic turning of the soil enhance degradation of brass crudeoil significantly. what is more, aeration by periodic turning slightly improves degradation only with cow dung treatment on days 21 and 28.