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15-Deoxy-Δ12,14-prostaglandin J2 Induces Apoptosis and Upregulates SOCS3 in Human Thyroid Cancer Cells
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نویسنده
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trindade-da-silva c.a. ,reis c.f. ,vecchi l. ,napimoga m.h. ,sperandio m. ,matias colombo b.f. ,alves p.t. ,ward l.s. ,ueira-vieira c. ,goulart l.r.
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منبع
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ppar research - 2016 - دوره : 2016 - شماره : 0
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چکیده
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The cyclopentenone prostaglandin 15-deoxy-δ12,14-prostaglandin j2 (15d-pgj2) is a natural ligand of peroxisome proliferator-activated receptor gamma (ppar-γ) and a potential mediator of apoptosis in cancer cells. in the present study,we evaluated the effect of 15d-pgj2 in human thyroid papillary carcinoma cells (tpc-1) using different doses of 15d-pgj2 (0.6 to 20 μm) to determine ic50 (9.3 μm) via the mtt assay. the supernatant culture medium of the tpc-1 cells that was treated either with 15d-pgj2 or with vehicle (control) for 24 hours was assessed for il-6 secretion via cba assay. rt-qpcr was used to evaluate mrna expression of il-6,socs1,socs3,and stat3. tpc-1 cells treated with 15d-pgj2 decreased the secretion and expression of il-6 and stat3,while it increased socs1 and socs3. overall,we demonstrated that 15d-pgj2 downregulated il-6 signaling pathway and led tpc-1 cells into apoptosis. in conclusion,15d-pgj2 shows the potential to become a new therapeutic approach for thyroid tumors. � 2016 carlos ant�nio trindade-da-silva et al.
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آدرس
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laboratory of nanobiotechnology,federal university of uberl�ndia,uberl�ndia,mg,brazil,laboratory of genetics,federal university of uberl�ndia,uberl�ndia,mg,brazil,hammock laboratory of pesticide biotechnology,university of california davis,davis,ca, United States, laboratory of nanobiotechnology,federal university of uberl�ndia,uberl�ndia,mg,brazil,laboratory of cancer molecular genetics,university of campinas,campinas,sp, Brazil, laboratory of nanobiotechnology,federal university of uberl�ndia,uberl�ndia,mg, Brazil, laboratory of immunology and molecular biology,s�o leopoldo mandic institute,research center,campinas,sp, Brazil, laboratory of immunology and molecular biology,s�o leopoldo mandic institute,research center,campinas,sp, Brazil, laboratory of nanobiotechnology,federal university of uberl�ndia,uberl�ndia,mg, Brazil, laboratory of nanobiotechnology,federal university of uberl�ndia,uberl�ndia,mg, Brazil, laboratory of cancer molecular genetics,university of campinas,campinas,sp, Brazil, laboratory of genetics,federal university of uberl�ndia,uberl�ndia,mg, Brazil, laboratory of nanobiotechnology,federal university of uberl�ndia,uberl�ndia,mg,brazil,department of medical microbiology and immunology,university of california davis,davis,ca, United States
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Authors
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