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New Transcriptional Reporters to Quantify and Monitor PPARγ Activity
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نویسنده
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degrelle s.a. ,shoaito h. ,fournier t.
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منبع
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ppar research - 2017 - دوره : 2017 - شماره : 0
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چکیده
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The peroxisome-proliferator-activated-receptor-γ (pparγ) is a member of the nuclear receptor superfamily that plays a critical role in diverse biological processes,including adipogenesis,lipid metabolism,and placental development. to study the activity of pparγ,we constructed two new reporter genes: a fluorescent gfp-tagged histone-2b (ppre-h2b-egfp) and a secreted nanoluciferase (ppre-pnl1.3[secnluc]). this study demonstrates their usage to monitor pparγ activity in different cell types and screen for pparγ's potential ligands. © 2017 séverine a. degrelle et al.
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آدرس
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inserm,umr-s1139,faculté de pharmacie de paris,paris,france,université paris descartes,sorbonne paris cité,paris,france,fondation premup,paris, France, inserm,umr-s1139,faculté de pharmacie de paris,paris,france,université paris descartes,sorbonne paris cité,paris, France, inserm,umr-s1139,faculté de pharmacie de paris,paris,france,université paris descartes,sorbonne paris cité,paris,france,fondation premup,paris, France
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Authors
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