Protective Effects of an Ancient Chinese Kidney-Tonifying Formula against H2O2-Induced Oxidative Damage to MES23.5 Cells

Oxidative damage plays a critical role in the etiology of neurodegenerative disorders including parkinson's disease (pd). in our study,an ancient chinese kidney-tonifying formula,which consists of cistanche,epimedii,and polygonatum cirrhifolium,was investigated to protect mes23.5 dopaminergic neurons against hydrogen peroxide- (h2o2-) induced oxidative damage. the damage effects of h2o2 on mes23.5 cells and the protective effects of ktf against oxidative stress were evaluated using mtt assay,transmission electron microscopy (tem),immunocytochemistry (icc),enzyme-linked immunosorbent assay (elisa),and immunoblotting. the results showed that cell viability was dramatically decreased after a 12 h exposure to 150 μm h2o2. tem observation found that the h2o2-treated mes23.5 cells presented cellular organelle damage. however,when cells were incubated with ktf (3.125,6.25,and 12.5 μg/ml) for 24 h after h2o2 exposure,a significant protective effect against h2o2-induced damage was observed in mes23.5 cells. using icc,we found that ktf inhibited the reduction of the tyrosine hydroxylase (th) induced by h2o2,upregulated the mrna and protein expression of ho-1,cat,and gpx-1,and downregulated the expression of caspase 3. these results indicated that ktf may provide neuron protection against h2o2-induced cell damage through ameliorating oxidative stress,and our findings provide a new potential strategy for the prevention and treatment of parkinson's disease. copyright © 2017 yihui xu et al.