Detection of islet cell cytoplasmic antibodies in first degree relatives of type-1 diabetic patients

Objectives: to estimate the islet cell cytoplasmic antibodies (ica) and insulin level in the sera of asymptomatic first degree relatives of patients with type 1 diabetes and compare with normal subjects. design of study: cross sectional comparative study data source: first degree relatives of patient with diagnosed type 1 diabetes attending various hospitals in lahore. setting: department of pathology,postgraduate medical institute,lahore. methodology: 120 subjects,60 asymptomatic first degree relatives of type 1 diabetics (group a) and 60 normal subjects were taken. the blood samples were collected in fasting state. 5 ml blood was collected and serum obtained was used for glucose,insulin and ica antibodies estimation. flu: results obtained from both groups were compared glucose was measured by oxidase method,insulin level by elisa method,and ica by indirect immune fluorecence method by commercially available kits. the students t test was applied for glucose and insulin level while chi square test was applied for ica to see the level of significance. conclusions: islet cell cytoplasmic antibodies (ica) are positive in 11 subjects (18.3%) of group a and 02 subjects (3.3:%) of group b.