Changing pattern of bacterial isolates from blood samples in paediatric ICUs

Objective: to observe most frequent bacterial isolate from septicemic children. setting: study was conducted in bmsi,jpmc,karachi with collaboration of tertiary care hospitals of karachi during july 2010 to december 2010. method: blood samples for culture were collected from 105 children admitted in icu with suspected septicemia. specimens were inoculated in brain heart infusion broth in ration of 1:5. subcultures were plerformed at 24 hours,72 hours and on the 7th day. isolates were identified by standard methods. results: a total of 67 organisms isolated out of 105 samples. these include gram negative (45/67) and gram positive (22/67). klebsiella were 18,staphylococcus aureus were 12,staphylococcus epidermidis were 10,proteus mirabilis 7,enterobacter 5,pseudomonas 5,escherichia coli 4,acinetobacter 3,citrobacter 2 and staphylococcus saprophyticus 1. conclusion: in the view of isolates klebsiella pneumoniae tends to show a higher frequency in the paediatric sepsis.