Re-interpretation of the single nucleotide polymorphism in the extracellular domain of tumor necrosis factor related apoptosis inducing ligand receptor at nucleotide 626 among ovarian cancer patients in Pakistani population

Objective: the study is focused on the snp in the extracellular domain of trail receptor dr4 at nucleotide 626 in ovarian cancer patients diagnosed in local population in pakistan. methods: 16 ovarian cancer patients and 23 control subjects participated in this study. 5ml venous blood was taken from participants with informed consent. dna was extracted using standard organic methods. pcr-rflp analysis was done for 626 c→g polymorphism in dr4 gene using site specific primers and restriction enzyme. the results were statistically evaluated in spss14. results: 16 ovarian cancer and 23 control samples were genotyped for c→g polymorphism in dr4 gene. the gg genotype was significantly lower in ovarian cancer patients and controls than the cc and cg in cancer patients and controls. conclusion: a detailed analysis in future studies and different cancers and cancer subtypes will provide a better understanding of the impact of snps in dr4 gene on its transcriptional status.