Biosurfactant production by pseudomonas aeruginosa strains on 1ml of inoculum size

Objective: to produce biosurfactants from pseudomonas aeruginosa using agricultural resource and to produce biosurfactants using low cost materials. study design: descriptive study place and duration of study: study was conducted at institute of molecular biology and biotechnology in university of lahore. duration of the study was two years. materials and methods: the volume of sample taken are 1ml,of innoculum from growing culture of pseudomonas aeruginosawas isolated from contaminated soil collected from industrial area of district kasoorand flasks were then placed into an orbital shaker at speed of 120rpm. the samples were collected in sterile screw capped bottle,4-5cm deep from the soil surface aseptically. the samples were stored at 4°c till further use. after every 24h the culture broth from each flask was taken to estimate bacterial cell mass. results:-surface tension was 61.8,48.1,33.7 and 31.9mn/m at time 24,48,72 and 96 hours respectively at constant temperature of 37°c and molasses used 0.25g with 1ml inoculum size. the rhamnolipid production was 0.61,1.24,2.13 and 2.74 g/l respectively. similarly the bacterial cell mass was 0.28,0.38,0.7 and 1.0g/l respectively. and the effect of inoculum size was also evaluated by the production of rhamnolipid as it is evident that at 1ml inoculum size 2.74g/l rhamnolipid was obtained after 96 hours . conclusion: optimizing various growth and environmental factors a production of rhamnolipid was achieved using 0.25g molasses with 1ml inoculum size.